An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering
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منابع مشابه
An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering
A novel method to link a nascent protein (phenotype) to its mRNA (genotype) covalently through the N-terminus was developed. The mRNA harboring amber stop codon at just downstream of initiation site was hybridized with hydrazide-modified ssDNA at upstream of coding region and was ligated to the DNA. This construct was then modified with 4-acetyl-phenylalanyl amber suppressor tRNA. This modified...
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ژورنال
عنوان ژورنال: International Journal of Biological Sciences
سال: 2007
ISSN: 1449-2288
DOI: 10.7150/ijbs.3.365